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. 2012 Nov 12;199(4):623–637. doi: 10.1083/jcb.201205114

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© 2012 TerBush and Osteryoung

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Figure 3. — Dynamics of FtsZ1 and FtsZ2 expressed singly or together. S. pombe cells expressing FtsZ1-eYFP (A, green; see also Video 1), FtsZ2-eCFP (B, red; see also Video 2), or FtsZ1-eYFP and FtsZ2-eCFP (C and D) were analyzed by FRAP. Images from left to right represent fluorescence signals in photobleached regions (circled) just before bleaching (PB), at the time of bleaching (0 s), at the time closest to t_1/2, and at twice t_1/2. Representative plots of fluorescence recovery vs. time are shown at right. Data in each plot were normalized to the PB fluorescence signal (1 on the y-axis) and the signal intensity at the time of bleaching (0 on the y-axis). Boxes in the plots show the average t_1/2 (also indicated by vertical lines) and average percent recovery ± SD for 10 independent FRAP datasets obtained for each strain. Bars, 2 µm.