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. 2012 Nov 12;199(4):599–611. doi: 10.1083/jcb.201207161

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© 2012 Klein et al.

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Figure 1. — Tob38, Tob37, and Tob55 are the subunits of the TOB complex. (A–D) OMVs from N. crassa with His-tagged Tob37 or Tob38 were solubilized with TX-100 (A and B) or digitonin (C and D). Proteins were isolated by Ni-NTA affinity purification and analyzed by SDS-PAGE followed by immunodecoration (A and B) or Coomassie blue staining (C and D). The calculated molecular weights for the proteins decorated are shown in A and B. The positions of molecular weight standards are shown in C and D. Solubilized OMVs before (S) or after (L) a clarifying spin. FT, flow through (proteins not bound to the Ni-NTA column); E, eluate of bound proteins. 55lg+int, Tob55-long and intermediate isoform; 55sh, Tob55-short isoform; 37, Tob37; 38, Tob38.