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. 2012 Nov 12;199(4):599–611. doi: 10.1083/jcb.201207161

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© 2012 Klein et al.

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Figure 2. — The native TOB complex. (A–D) The TOB complex was isolated from cells containing either His-tagged Tob38 or Tob37 using TX-100 (A and B) or digitonin (C and D). BHM were solubilized with TX-100 (A and B) or digitonin (C and D). The samples were analyzed by BNGE and immunodecoration (A and C) or Coomassie blue staining (B and D). Asterisks indicate possible oligomeric forms of the TOB complex. (E) The TOB complex remains intact during the isolation of OMVs from mitochondria. Mitochondria (M) and OMVs (O) from either wild-type (wt) N. crassa or strains bearing His-tagged Tob55 (55), Tob38 (38), or Tob37 (37) were solubilized with digitonin. They were then analyzed by BNGE and immunodecorated with Tob38 antiserum. (A–E) Membrane protein complexes from BHM were used as marker proteins for relative molecular weights.