Table 1.
Sample | No. of micrographs | No. of particles picked | Particles in final reconstruction | Resolution FSC 0.5 |
His9-Tob37 | 546 | 175,000 | 76,700 | 14.7 |
His9-Tob37 gold labeleda | 415 | 155,000 | 88,000 | 15.0 |
His9-Tob38 | 318 | 104,000 | 43,500 | 15.0 |
His9-Tob38 gold labeleda | 125 | 45,000 | 25,900 | 15.6 |
His9-Tob55 | 215 | 80,000 | 40,600 | 14.7 |
His9-Tob55 gold labeleda | 272 | 98,500 | 46,400 | 14.5 |
Dimers from His9-Tob55 | N/A | 24,800 | 19,300 | 13.7 |
Although no gold label could be detected for His9-Tob37 and His9-Tob38, the structures are identical with or without added Ni-NTA nanogold.