Figure 3.
Lentivirus-mediated conditional disruption of mGluR5 in the central amygdala is analgesic. A, Representative Western blot for mGluR5 (top) and β-tubulin (bottom) in mGluR5CeAKO and WT LV-Cre-injected (control) mice. Graph of mGluR5/β-tubulin shows reduced mGluR5 expression in the right amygdala of mGluR5CeAKO mice (n = 8) compared with the right amygdala of control mice (n = 8). B, Disruption of mGluR5 in the right amygdala (n = 8) induced a significant decrease in evoked response during bladder distention compared with control mice (n = 9). Brain atlas insets show cannula targeting with black squares indicative of cannula tips of control mice and gray triangles indicative of cannula tips of mGluR5CeAKO mice (***p < 0.001, two-way ANOVA main effect of genotype; **p < 0.01, Student's t test). Error bars indicate SEM.