Table 6.
List of used antibodies
| A: Antibodies for PLA analysis | |||
|---|---|---|---|
| Antibodies | References | Species | Dilution |
| ERα (1D5) | 7047 (Dako) | Mouse | 1/50 |
| ERα (HC20) | Sc-542 (Santa Cruz) | Rabbit | 1/75 |
| PI3K p85 | Ab22653 (Abcam) | Mouse | 1/30 |
| Src (B12) | Sc-8056 (Santa Cruz) | Mouse | 1/150 |
| mERα | Home made | Mouse | 1/50 |
| p300 (NM11) | Sc-32244 (Santa Cruz) | Mouse | 1/100 |
| SRC3 | Sc-13066 (Santa Cruz) | Rabbit | 1/50 |
| FAK (A17) | Sc-557 (Santa Cruz) | Souris | 1/50 |
| B: Antibodies for immunoprecipitation and Western blotting | ||||
|---|---|---|---|---|
| Antibodies | References | Species | Dilution Western Blotting | IP |
| mERα | Home made | Mouse | 1 µg | |
| ERα (60C) | #04-820 (Millipore) | Rabbit | 1/1000 | |
| PRMT1 | #24-333 (Upstate) | Rabbit | 1/4000 | |
| PI3K p85 | #06-195 (Upstate) | Rabbit | 1/9000 | |
| c-Src (B12) | Sc-8056 (Santa Cruz) | Mouse | 1 µg | |
| Src (36D10) | #2109 (Cell signaling) | Rabbit | 1/1000 | |