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. 2012 Oct 4;109(45):18517–18522. doi: 10.1073/pnas.1215928109

Fig. 2.

Fig. 2.

ANi2.3 TCR binds to mimotope–DR52c complexes with typical TCR kinetics. The results of surface plasmon resonance studies are shown for the binding of soluble (A) DR52c–pHIR and (B) DR52c–pWIR complexes to immobilized ANi2.3 TCR. The binding kinetics were recorded in resonance unit (RU) when four different concentrations of DR52c–mimotope complexes were injected for 80 s through a flow cell of a Biacore CM5 biosensor chip containing immobilized ANi2.3 TCR. Identical injections in a second flow cell with an immobilized control TCR (Yae62) were used to correct for the fluid phase signal. Association rates (kas), dissociation rates (kds), and overall (affinity) KD (dissociation constant) with SEM were calculated from the kinetic data using instrument software.