Table II.

Components of the carrot eEF-1A PGE posttranslational modification

Fraction	[14C]Ethanolamine		[3H]Glycerol		[14C]Myristate
	Initial aliquot	Delipidated product	Initial aliquot	Delipidated product	Initial aliquot	Delipidated product
	dpm	dpm/mg protein	dpm	dpm/mg protein	dpm	dpm/mg protein
Supernatant	3.8  × 105	2.8 × 104  ± 2.8 × 103	2.8  × 105	1.7 × 104  ± 4.3 × 102	5.2  × 105	1.2 × 105  ± 1.7 × 104
Purified eEF-1A	1.2  × 107	6.9 × 104  ± 8.1 × 103	4.6  × 105	5.6 × 104  ± 1.3 × 104	4.4  × 105	1.8 × 104  ± 4.8 × 103
Microsomes	3.1  × 105	6.4 × 104  ± 6.3 × 103	3.1  × 105	3.7 × 104  ± 3.1 × 103	2.5  × 106	2.5 × 105  ± 2.1 × 104

Carrot cells were labeled with [¹⁴C]ethanolamine, [¹⁴C]myristic acid, and [³H]glycerol as described in Methods. Supernatant (40,000g), microsomal pellet, and purified eEF-1A fractions were TCA precipitated and delipidated. The numbers for the delipidated protein represent the average of three samples.