Fig. 4.

HMM scFv recovery strategy and FACS staining. (A) Candidate HMM scFv clones are recovered by PCR using primers specific for L3 and H3, and which also have 5′ homology arms for subsequent isothermal assembly into an expression vector with differing codon use. (B) Results of FACS staining to assess binding of candidate scFvs. HMECs infected with vector alone or vector-expressing PVRL4 antigen were stained with the indicated control antibodies or one of the top four most abundant postselection HMM scFv clones.