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Total synthesis of HMM scFv clones. Strategy for reconstruction of desirable HMM scFv clones from synthetic oligos. After PCR amplification of the oligo or oligo library, the CDRs are assembled into an intermediate expression vector. The constant sequences of the framework regions between L3 and H2 (green) and between H2 and H3 (purple) are then sequentially inserted to form the complete HMM scFv expression clone or clone set.
