Fig. 3.

Direct IL-27 signaling is required for IL-10 expression. (A and B) Mixed bone marrow chimeras comprising WT and IL-27Rα^−/− Vert-X cells were infected with Sendai virus and, 10 d later, cells from the bronchoalveolar lavage (BAL) and lung were analyzed by flow cytometry. (A) IL-10 expression; (B) frequency of Sendai-specific cells. (C) Naïve CD8+ T cells from WT and IL-27Rα^−/− Vert-X mice were labeled with a violet proliferation dye, VPD450, and stimulated in vitro with anti-CD3 and anti-CD28 in the presence or absence of recombinant IL-27. Cells were analyzed by flow cytometry 3 d later. In all panels, data shown are gated on CD8+ lymphocytes and representative of three independent experiments. Graphs depict mean ± SD of five mice per group.