Fig. 2.

Nuclear targeting of TPs from diverse phages. Confocal images of representative COS-7 cells expressing the indicated 2YFP fusions to wild-type Φ29, Nf, GA-1, PRD1, Bam35, Cp-1, and ABV TPs, selected among at least five pictures. Shown are YFP (green), DAPI (red), and YFP merged with DAPI images. The 2YFP–ABV construct gave rise to a disperse signal throughout the cell and a large focus of strong signal that might correspond to the aggresome formation reported for similar chimeric fusion proteins (54), although no significant degradation is observed in a Western blot to monitor the expression and stability of YFP fusions (Fig. S5).