Fig. 5.

Targeted heterozygous Cxcr4 mutation selectively modifies interneuron migration in the developing LgDel cortex. There is a visible difference in the distribution of Dlx5/6-CIE interneurons in the VZ/SVZ of (A) E13.5 WT/Dlx5/6-CIE/Cxcr4^wt/f, (B) LgDel, and (C) LgDel/Dlx5/6-CIE/Cxcr4^wt/f cortex. (D) Tangential interneuron progress into cortex was quantified between the cortical/subcortical boundary and the cortical hem. There was an equivalent, significant reduction of tangential migration in the LgDel and LgDel/Dlx5/6-CIE/Cxcr4^wt/f embryos. (E) Interneuron distribution was quantified in six equidistant bins between the pial and ventricular surfaces. Migratory interneuron frequency in the VZ of the LgDel is increased relative to WT (WT/Dlx5/6-CIE/Cxcr4^wt/f; LSD test, P = 0.045 at bin location 5), and this altered frequency is increased further in the LgDel/Dlx5/6-CIE/Cxcr4^wt/f (LSD test; P = 0.003 at bin location 5). Furthermore, there is a significant increase in interneurons in the VZ of the LgDel/Dlx5/6-CIE/Cxcr4^wt/f over the LgDel (LSD test; P = 0.05; error bars represent SEM).