Fig. 1.

Design of recombination cassettes. The schematic represents the design of the ΔβCDR3 vector incorporating a recombination cassette in the germ-line CDR2 (A) or CDR1 (B). The constructs also contain a minimal triple-glycine CDR3 region flanked by framework V- and J-segment residues (13). The general design (Left) and details of the protein and nucleotide sequence (Right) are shown. The arrow represents the point of recombination. (C) Insertion of a recombination cassette into the CDR1 of a TCR-β chain with all three CDRs replaced with glycine-alanine linkers. Schematics are presented as for B. Highlighted residues represent those mutated from the original template.