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. 2012 Sep 27;109(45):E3111–E3118. doi: 10.1073/pnas.1210882109

Fig. 6.

Fig. 6.

TCR-γ germ-line CDRs mediate αβ T-cell selection. (A) Schematic depiction of hybrid TCR chain containing germ-line CDR1/2 derived from TCR Vγ1 (construct βCDR1γ/2γ/3∆) grafted onto TCRVβ11 (framework Y54 deleted and minimal triple-glycine CDR3). (B) CD5 expression on GFP+ DP (solid line plots) and double-negative (dashed line plot) thymocytes from WT (TCR-β+δ+) FVB/n (Lower) and βCDR1γ/2γ/3∆ (Upper; n = 4). (C) Representative plots and summary charts of splenic and lymph node GFP+, CD4, and CD8 T-cell representation in βCDR1γ/2γ/3∆ retrogenic mice. (D) Representative plots and summary charts of CD44hi GFP+ CD4 and CD8 T cells from whole spleen in βCDR1γ/2γ/3∆ retrogenic mice. Compare with Fig. 3C and Fig. S3D.