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Inhibition of FKHD-mediated gene expression by tert-Butylhydroquinone (tBHQ). Cortical neurones (8 DIV) were transfected with pARE-luc (a, b) or FHRE-luc (c, d) and then treated with, 10 μM or 30 μM tBHQ for 24 h (a and c) or 30 μM tBHQ for different time periods as shown (b and d). Data represent mean ± SEM, analysed using one-way anova and Bonferroni post hoc test (n = 3, each concentration performed in triplicate) where *p < 0.05, **p < 0.01 ***p < 0.001 compared to vehicle control.
