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. 1998 Jul;117(3):961–970. doi: 10.1104/pp.117.3.961

Figure 2.

Figure 2

The degradation of exogenous LHCII as affected by the concentration of thylakoid membrane protein in assay mixtures (A) or the concentration of exogenous LHCII added (B). A, Exogenous LHCII apoprotein (5 μg) was added to increasing amounts of TX-100-solubilized PLB protein obtained from 8-d-old etiolated plants in assay mixtures of 35 μL (0.5% TX-100, 40 mm Tris HCl, pH 8.6). B, To nonsolubilized primary thylakoid protein (40 μg) obtained from 6-d-old etiolated plants kept in the dark for 48 h after a 2-min light pulse, increasing amounts of exogenous LHCII apoprotein were added in assay mixtures of 48 μL (0.25% TX-100, 40 mm Tris-HCl, pH 8.6). Proteolytic activity is based on the amount of exogenous LHCII apoprotein remaining after 45 min (A) or 15 min (B) of incubation at 37°C as monitored by immunodetection on western blots.