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. 1998 Jul;117(3):961–970. doi: 10.1104/pp.117.3.961

Figure 3.

Figure 3

Specificity of the thylakoid-bound proteolytic activity. Primary thylakoid protein (40 μg) from 6-d-old etiolated plants exposed to ImL for 16 LDCs, isolated by differential centrifugation, were incubated at 37°C with 8 μg of exogenous LHCII apoprotein (immunodetection with anti-LHCII); without addition (immunodetection with the anti-β-subunit of coupling-factor 1 [β-CF1] or anti-POR); with 8 μg of BSA or lactoperoxidase (Coomassie blue stained); or with 16 μg of protein of a stroma fraction from 10-d-old etiolated intact plastids (immunodetection with anti-LSU). All assay mixtures had a final volume of 48 μL and a final TX-100 concentration of 0.05%.