Skip to main content
. 1998 Jul;117(3):961–970. doi: 10.1104/pp.117.3.961

Figure 4.

Figure 4

The effect of NaBr washing on the proteolytic activity of thylakoids and the release of the activity in the wash. Thylakoids were obtained by differential centrifugation from 6-d-old etiolated plants exposed to CL for 4 d. After NaBr washing, the thylakoid pellet (T) was solubilized in TX-100, and the dialyzed supernatant (W) was lyophilized. The TX-100-solubilized thylakoid protein (80 μg in 80 μL) or the lyophilized wash protein (90 μg mixed with 15 μg of exogenous LHCII in 105 μL) were incubated at 37°C. All assay mixtures contained final concentrations of 0.5% TX-100 and 40 mm Tris-HCl, pH 8.6.