Figure 5.

The effect of the etiolated tissue age on the proteolytic activity of PLBs isolated by differential centrifugation. TX-100-solubilized protein (120 μg) was mixed with 20 μg of exogenous LHCII apoprotein in a final volume of 140 μL. The assay mixture also contained 0.5% TX-100 and 40 mm Tris-HCl, pH 8.6, and was incubated at 37°C for 1 h.