Figure 6.

Proteolysis of exogenous LHCII apoprotein by nonsolubilized thylakoids isolated by differential centrifugation at various stages of development. Six-day-old etiolated plants were kept in the dark (▴) or exposed to a 2-min white-light pulse and kept in the dark thereafter (○) or to ImL (2 min of light, 98 min of dark) (•). To 40 μg of PLB or primary thylakoid protein, 8 μg of exogenous LHCII apoprotein was added to assay mixtures of 48 μL (0.05% TX-100, 40 mm Tris-HCl, pH 8.6) and incubated for 30 min at 37°C.