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Accumulation of D1 in primary thylakoids of 6-d etiolated plants exposed to 20 LDCs isolated by differential centrifugation, as affected by the dark-phase duration in ImL. One-hundred micrograms of nonsolubilized thylakoid protein was loaded per slot. Accumulation is estimated on the basis of the area under the D1 curve, as monitored by scanning of immunoblots.
