Table III.

Effect of the addition of Chl-rich mature thylakoids obtained from CL plants on the proteolytic activity of Chl-deficient primary thylakoids of etiolated plants exposed to a pulse of light and kept in the dark for 50 h thereafter

Sample	Endo LHCII	Exo LHCII	Endo + Exo LHCII	LHCII Degradeda
	μg
Primary	–	6	6.0	6.0
Mature	14.8	–	14.8	5.4
Primary + mature	14.8	6	20.8	12.5

Primary thylakoids were obtained from 6-d-old etiolated bean plants exposed to a 2-min white-light pulse and then kept in the dark for 50 h. Mature thylakoids were obtained from 6-d etiolated bean plants exposed to CL for 72 h. Primary thylakoids were not solubilized. Mature thylakoids were solubilized in TX-100. Final volume of the assay mixtures was 66 μL, and the final TX-100 concentration was 0.5%. The assays had either 30 μg of primary thylakoid protein mixed with 6 μg of exogenous (Exo) LHCII apoprotein, 30 μg of mature thylakoid protein containing 14.8 μg of endogenous (Endo) LHCII apoprotein, or a mixture of both.

^aDegradation for 30 min at 37°C.