Table V.

Comparison of proteolytic activity of primary thylakoids from ImL plants having similar Chl accumulation or similar developmental stage

Time in Cycles	No. of Cycles	LHCII Degradeda		Chl a	Chl b
		%	μg
h				μg g−1 fresh wt
7	18 (2 min of light, 23 min of dark)	8	1.6	142	21
24	18 (2 min of light, 83 min of dark)	38	7.6	187	7
24	59 (2 min of light, 23 min of dark)	33	6.7	463	89

Six-day-old etiolated plants were exposed to LDCs with short or long dark intervals. Primary thylakoids were isolated by differential centrifugation, washed twice with 50 mm Tricine, pH 7.3, and solubilized in TX-100. TX-100-solubilized primary thylakoids (120 μg) were mixed with 20 μg of exogenous LHCII apoprotein in a final volume of 140 μL (0.5% TX-100, 40 mm Tris-HCl, pH 8.6). Ten micrograms of protein (thylakoid protein plus exogenous LHCII apoprotein) was applied to gels.

^aDegradation at 37°C for 30 min.