Figure 3.

Western blot analysis of expressed MCCC2 wildtype and mutant proteins. Constructs with MCCC2 wildtype and 8 mutant cDNAs in pTracer vector were transfected into MCCC2 deficient reference cell lines by electroporation and harvested 48 hours later for Western blot analysis. 50 μg of protein were used per lane. The MCCC2 subunit was visualized by immunostaining using β-actin (6 μg) as control. Transfection with an empty vector (vector only) was used as a negative control. For further details see “Methods”.