Table 1.
Animals for Experiments on Temporal Pattern Changes Following Diffuse Brain Injury
| Outcome | Experiment | Group | Time point (d) |
N | Weight (g) (mean ± SD) |
Apnea (sec) (mean ± SD) |
|---|---|---|---|---|---|---|
| Axonal conduction | Time course | Sham | 1 | 6 | 34 ± 2 | NA |
| Sham | 7 | 8 | 33 ± 3 | NA | ||
| Sham | 14 | 7 | 31 ± 3 | NA | ||
| Injured | 1 | 8 | 35 ± 3 | 6 ± 1 | ||
| Injured | 7 | 8 | 35 ± 3 | 7 ± 2 | ||
| Injured | 14 | 7 | 33 ± 2 | 6 ± 1 | ||
| Neurofilament dephosphorylation (Immunohistochemistry) |
Time course from (19) |
Sham | 1 | 4 | 36 ± 2 | NA |
| Sham | 3 | 4 | 36 ± 3 | NA | ||
| Sham | 7 | 4 | 38 ± 2 | NA | ||
| Injured | 1 | 4 | 35 ± 2 | 5 ± 0 | ||
| Injured | 3 | 4 | 37 ± 2 | 7 ± 3 | ||
| Injured | 7 | 4 | 35 ± 1 | 6 ± 2 | ||
| Neurofilament expression (Western blots) |
Time course | Sham | 1 | 5 | 34 ± 4 | NA |
| Sham | 3 | 4 | 36 ± 6 | NA | ||
| Sham | 7 | 5 | 34 ± 3 | NA | ||
| Injured | 1 | 7 | 38 ± 4 | 6 ± 1 | ||
| Injured | 3 | 7 | 34 ± 4 | 7 ± 3 | ||
| Injured | 7 | 7 | 36 ± 4 | 6 ± 2 | ||
| Calcineurin activation | Time course | Sham | 1 and 3 |
8 | 36 ± 5 | NA |
| Injured | 1 | 10 | 35 ± 5 | 5 ± 2 |
The animals used to determine the pattern of neurofilament dephosphorylation were generated in an earlier study (19). NA = not applicable.