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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Biochemistry. 2012 Sep 25;51(40):8003–8013. doi: 10.1021/bi300781g

Table 1.

Equilibrium association constants (KAS) for the complexes of mononucleotide cap analogs with the human and nematode DcpS, obtained from analysis of steady-state fluorescence titration at 20 °C, in 50 mM Tris HCl buffer containing 200 mM KCl, 0.5 mM EDTA and 1 mM DTT (final pH 7.6); nd – not determined.

Cap analog KAS [μM−1]
HsDcpS AsDcpS CeDcpS
m7GMP 19 ± 1 26.35 ± 1.84 8.00 ± 0.65
m7GDP 114 ± 8 185.13 ± 16.48 97.04 ± 12.52
m32,2,7GMP nd 0.28 ± 0.27 2.88 ± 0.67
m32,2,7GDP nd 0.53 ± 0.16 1.44 ± 0.26