Table 4.

Detection of MV RNA in biological fluids/excretions by RT-qPCR at day 11

Treatment group		Detection of viral RNA				Result infectivity assay
		Signal < LODa		Positive signal (+)b
		Animals (N)	Body fluid	Animals (N)	Body fluid
NaCl	Group 1	–	–	–	–	–
	Group 4	–	–	–	–	–
MV1-F4	Group 2	2 M	Faeces	–	–	Negative
	Group 5	1 F	Urine, vaginal secretion	–	–	Negative
	Group 7	–	–	–	–	–
Rouvax	Group 3	1 F	Nasal swab	–	–	Negative
	Group 6	1 M	Serum, faeces, PBMCs	1 M	Throat swab	Negative
		1 M	Nasal swab, faeces
		1 F	Throat swab, vaginal secretion
	Group 8	1 MM	PBMCs	–	–	Negative

(M)M/F (mature) male/female; N no. of animals with positive signal in sample/no. of tested animals in that group

^a<LOD: RNA signal detected but the number of copies was less than the defined LOD of 100 genome equivalent (geq)/reaction (with 95 % chance to detect a positive signal).

^b+: positive signal, i.e., signal > defined LOD of the RT-qPCR assay (with 95 % probability)