Table 5.
Detection of MV RNA in organs and tissues by RT-qPCR
| Organs/tissues | Day 11 | Day 85 | ||
|---|---|---|---|---|
| MV1-F4 — 1 dose (Group 2) N | Rouvax — 1 dose (Group 3) N | MV1-F4 — 3 doses (Group 5) N | Rouvax — 3 doses (Group 6) N | |
| Adrenals | 1/6 < LOD | 1/6 < LOD | – | – |
| Large intestine (caecum/colon/rectum) | 2/6+; 2/6 < LOD | 3/6+; 1/6 < LOD | – | 1/6+ |
| Small intestine (ileum/jejunum/duodenum) | 2/6+; 1/6 < LOD | 4/6+; 1/6 < LOD | – | 1/6 < LOD |
| Eye: retina + lid | 1/6 < LOD | 1/6 < LOD | – | – |
| Gall bladder | – | 2/6+ | – | – |
| Kidneys | – | 1/6+ | – | – |
| Larynx | 1/6+ | 2/6+; 1/6 < LOD | – | 1/6 < LOD |
| Liver | 3/6 < LOD | 1/6+; 5/6 < LOD | – | – |
| Lungs with bronchi | 1/6+; 1/6 < LOD | 1/6 < LOD | – | – |
| Lymph nodes (cervical) | 4/6+ | 5/6+ | 1/6+ | 2/6+; 1/6 < LOD |
| Lymph nodes (iliac) | 4/6+; 1/6 < LOD | 4/6+ | 3/6+; 1/6 < LOD | 2/6+; 2/6 < LOD |
| Lymph nodes (mandibular) | 4/6+ | 5/6+ | 2/6 < LOD | 4/6+ |
| Lymph nodes (mesenteric) | 3/6+; 1/6 < LOD | 5/6+ | 1/6 < LOD | 3/6+; 2/6 < LOD |
| Lymph nodes (popliteal) | 2/6 < LOD | 2/6+; 1/6 < LOD | 1/6+ | 3/6+ |
| Mammary gland area | – | 1/6 < LOD | – | – |
| Mesenteric artery | – | 1/6+; 1/6 < LOD | – | – |
| Pancreas | – | 2/6 < LOD | – | – |
| Peyer’s patches | 2/6+; 1/6 < LOD | 4/6+ | – | 1/6+; 1/6 < LOD |
| Salivary glands | 2/6 < LOD | – | – | – |
| Skin | – | 1/6 < LOD | – | – |
| Pia maters (cervical/lumbar/thoracic) | – | 1/6 < LOD | – | – |
| Spleen | 4/6+; 1/6 < LOD | 5/6+ | 1/6+; 2/6 < LOD | 3/6+; 1/6 < LOD |
| Sternum with bone marrow | – | 2/6 < LOD | – | – |
| Thymus | – | 1/6+ | – | – |
| Tongue | – | 1/6 < LOD | – | – |
| Trachea | 2/6 < LOD | 2/6+; 1/6 < LOD | – | 1/6 < LOD |
| Ureters | 1/6+ | – | – | – |
| Urinary bladder | 1/6+; 2/6 < LOD | 1/6 < LOD | – | – |
| Uterus corpus + cervix | – | 1/3 < LOD | – | – |
| Vagina | – | 1/3 < LOD | – | – |
No detections were made in the remaining organs (aorta, brain, cerebellum, cerebrum, epididymides, femoral bone with articulation, heart, hypothalamus, injection sites, knee joint, optic nerve, oesophagus, ovaries, oviducts, pituitary gland, prostate, sciatic nerve, seminal vesicles, skeletal muscle, spinal cord regions, stomach (fundus), testes, thalamus, thyroids and parathyroids) for these groups and time points
N no. of animals with positive signal in tissue sample/no. of tested animals in that group; +/−: + = positive signal (signal > defined limit of detection [LOD] of the RT-qPCR assay [with 95 % probability]). – : no RNA signal detected; <LOD: RNA signal detected but the number of copies was less than the defined LOD of 100 geq/reaction (with 95 % chance to detect a positive signal)