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Effect of pH on specific [14C]spermidine binding to total proteins solubilized from zucchini PM vesicles. The assay was performed with Tris-Mes buffer from pH 5.5 to 7.0 (▴) and with Tris-HCl buffer from pH 7.0 to 9.0 (▪). The values are means ± sd of triplicate samples from two replicate experiments. prot, Protein.
