FIG. 2.
Temporal profile of GABAA α1 and α4 receptor subunits after fluid percussion injury (FPI). (A) Representative Western blots from whole hippocampi of rats 24 h, 48 h, and 1 week after FPI probed with anti-GABAAR α1 and β-actin antibodies. (B) Quantification of α1 blots showed a significant decrease at 24 h through 1 week after FPI injury relative to controls. α1 Levels were normalized to β-actin levels and expressed as percent change compared to sham-injured controls. (C) Representative Western blots from whole hippocampi of rats 24 h, 48 h, and 1 week after FPI probed with anti-GABAAR α4 and β-actin antibodies. (D) Quantification of α4 blots showed a significant increase at 24 h after FPI, but at 1 week there was a significant decrease relative to controls. α4 Levels were normalized to β-actin levels and expressed as percent change compared to sham animals (24 h: n=7 for sham, 6 for FPI; 48 h: n=6 for sham, 8 for FPI; 1 week: n=7 for sham, 7 for FPI; GABAAR, gamma-aminobutyric acid type A receptor).