FIG. 2.

Expression of ZsGreen and hCAST in RGCs and their axons within the optic nerve. Rats were perfusion fixed 3 weeks after intravitreal injection of AAV.ZsGreen or AAV.hCAST. Representative images of retinal flat mounts immunolabeled with MAB3084 (hCAST), and/or an antibody directed against neuron-specific class III β-tubulin that is used to label RGCs (PRB-435P; scale bar=20 μm). (B) Representative images of longitudinal sections of prechiasmatic optic nerves immunolabeled with MAB3084 (scale bar=20 μm). (C) Western blot confirmed hCAST expression in all optic nerves after intravitreal injection of AAV.hCAST (n=10; hCAST, human calpastatin; RGC, retinal ganglion cell; GAPDH, glyceraldehyde 3-phosphate dehydrogenase). Color image is available online at www.liebertonline.com/neu