FIG. 2.

OM-MSCs promote the proliferation and survival of human BMCs. NACs were collected from BMC/OM-MSC co-cultures and the cell number and viability were determined by trypan blue technique. Results show an increase in the number of NACs at 54 days with respect to 13 days of co-cultures (A). Cell viability was >80% at 54 days of co-culture (B). Microscopic evaluation shows proliferation of rounded cells on OM-MSC monolayer, at 13 and 41 days of BMC/OM-MSC co-cultures (C). Results are representative of at least 3 experiments, all with similar results. BMCs, bone marrow cells; NACs, nonadherent cells.