FIG. 5.

Detection of CFU-GM progenitors in BMC/OM-MSC co-cultures. NACs were collected from BMCs/OM-MSCs, after 17 days of co-culture, and colony-forming assays were performed. Microscopic evaluation shows the presence of a CFU-GM colony (A). Single CFU-GM colonies were taken from methylcellulose assays and subjected to cytospin, and May-Grünwald/Giemsa staining showing the presence of myeloid cells (B). Flow cytometry analysis of NACs shows the presence of cells expressing the CD34 marker (C). Results correspond to 1 experiment representative of 2 different assays, all with similar results. CFU-GM, colony-forming unit–granulocyte/macrophage.