FIG. 4.

Forced expression of asb11 mRNA increases muscle compartment size. (A) Zebrafish zygotes were injected at the 2-cell stage with encoding full-length MT-d-asb11 mRNA at different concentrations or 200 pg of GFP-encoding mRNA as a control. Injection of increasing amounts of d-Asb11 mRNA (50, 100, 150, and 200 pg) in zygotes causes CNS and especially brain hypertrophy, which was described earlier at 48 h [12], but also the muscle compartment appears enlarged. Ob, olfactory bulb; Te, telencephalon; Cb, cerebellum; Am, adaxial myotomes; Ey, Eye; Yo, yolk sac; CNS, central nervous system. Bar indicates 100 μm. (B) Morphometric analysis of the adaxial myotome hemicircumference at equivalent position in the embryo is consistent with an increased muscle compartment size following d-Asb11 misexpression. Color images available online at www.liebertpub.com/scd