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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Prostaglandins Leukot Essent Fatty Acids. 2012 Sep 10;87(4-5):127–134. doi: 10.1016/j.plefa.2012.08.003

Figure 4. Aspirin-triggered lipoxin (ATL) reduces leukocyte-endothelial interactions initiated by preeclamptic plasma, 8-isoprostane and TBARS: Endothelial and leukocyte directed actions.

Figure 4

HUVEC cells were stimulated overnight with IL-1β (positive control, 10 ng/mL), plasma from normotensive pregnant women (n=4) or preeclamptic women (n=8). Then, PMN labeled with CFDA-SE were added to pre-stimulated HUVEC cells, and cell adhesion was assessed after 15min as described in methods (A). On the other hand, HUVEC were stimulated overnight with 8-isoprostane (0.1–1000 nM) (C) and TBARS (1–100.000 nM) (E). Human PMN were incubated with ATL (10nM; 15min) prior to addition to stimulated HUVEC monolayers with plasma, 8-isoprostane and TBARS, respectively (B,D,F). Results are mean ± s.e.m *P<0.05, **P<0.01,***P<0.001