Table I.
Effect of various concentrations of (+)-ABA on VLCMFA accumulation
| (+)-ABA Concentration | VLCMFA Content
|
|||||
|---|---|---|---|---|---|---|
| 20:1 | 22:1 | 20:1 + 22:1 | ||||
| μm | μg mg−1 protein | mol % | μg mg−1 protein | mol % | μg mg−1 protein | mol % |
| 0 | 179 ± 9 | 10 | 220 ± 15 | 11.2 | 399 | 21.2 |
| 1 | 206 ± 15 | 11.6 | 279 ± 8 | 13.7 | 485 | 25.3 |
| 10 | 273 ± 26 | 13.4 | 377 ± 24 | 16.1 | 650 | 29.5 |
| 15 | 277 ± 21 | 12.9 | 345 ± 17 | 15.2 | 622 | 28.1 |
| 33 | 261 ± 19 | 12.2 | 313 ± 20 | 14.3 | 574 | 26.5 |
| 100 | 250 ± 10 | 12.1 | 292 ± 11 | 14.6 | 542 | 26.7 |
Microspore-derived B. napus embryos were incubated with various concentrations of (+)-ABA in culture. After 48 h of treatment, embryos were harvested, total lipids isolated, and fatty acid composition determined as described in Methods. The data shown are means ± se of four replicates.