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. 2012 Aug 20;10:59. doi: 10.1186/1477-7827-10-59

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Copyright ©2012 Kouba et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Percent sperm motility from male Anaxyrus americanus treated with either hCG (Panel A) or LHRH (Panel B) over time after administration with either: 100 (triangles), 200 (squares), or 300 (diamonds) IU hCG compared to 0.1 (circles), 1.0 (triangles), 4 (squares) or 32 (diamonds) μg LHRH. Forward Progression for these same sperm samples are shown in Panel C for hCG or Panel D for LHRH. Only one male American toad administered 50 IU hCG produced sperm for quality assessment and was not included in Panel A and C. Data are expressed as Mean ± SEM; lower SEM bars not shown. For number of animals comprising each treatment Mean see Figure 1.