Skip to main content
. Author manuscript; available in PMC: 2012 Nov 11.
Published in final edited form as: Methods Cell Biol. 2010;97:15–33. doi: 10.1016/S0091-679X(10)97002-7

Figure 5. +TIPs as reporters of MT polymerization dynamics.

Figure 5

(A) Images of EGFP-tagged EB1 from the same dual-wavelengths time-lapse sequence as in Fig. 2. Scale bar, 5 um. (B) Computer-generated growth tracks of the same two MTs as in Fig. 2 with shortening events (light grey arrows) inferred by geometrical cluster analysis. (C) Maximum intensity projection of the entire image sequence directly showing EB1-EGFP growth tracks. (D) Computer-generated growth tracks with a minimum life-time of four frames demonstrating high tracking fidelity.