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. Author manuscript; available in PMC: 2012 Nov 12.
Published in final edited form as: Science. 2011 Jul 21;333(6047):1300–1303. doi: 10.1126/science.1210597

Figure 1. Optimization of conditions for detection of cytosine and its 5-position modified forms by TLC.

Figure 1

(A)Migration of labeled C and its 5-position modified forms by TLC under the first developing buffer. Lanes 1-3 serve as controls for the migration of 5mC and 5hmC generated from DNA oligos incubated with wild-type or catalytic mutant Tet2.

(B) The same samples used in panel A were separated by TLC under the second developing buffer. With the exception of 5mC and C, all of the other forms of C can be separated under this condition.

(C) Autoradiographs of 2D-TLC analysis of samples derived from 5mC-containing TaqI 20mer oligo DNA incubated with wild-type and catalytic-deficient mutant Tet1, Tet2, and Tet3.