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Myotube formation and desmin expression of periodontal ligament (PDL) stromal cells. Control cells cultured in the absence of 5-aza-2'-deoxycytidine (5-Aza) treatment, A and cells pretreated with 5-Aza for DNA demethylation, B. Myotube-like structures that were positive for desmin (B, green) were only observed in B. Cell nucleus counterstained with DAPI (blue).
