Table 1.

Richness and diversity estimation of the 16S rRNA gene libraries from the pyrosequencing analysis.

Sample	Number of reads	Number of OTUsa	Species richness indices			Species diversity indices
			Chao1	ACE	Jackknife	Shannon	Simpson
Eggs	1 798	28	28	28	28	2.62	0.88
Early-instar larvae	86 038	21	21	21	21	0.87	0.37
Third-instar larvae	112 382	71	71	71	71	1.40	0.58
Pupae	108 609	30	30	30	30	1.47	0.70
3- to 7-day-old adult males	113 614	19	19	19	19	1.26	0.59
3- to 7-day-old adult females	159 309	15	15	15	15	0.35	0.15
3- to 5-week-old adult males	85 095	17	17	17	17	0.72	0.32
3- to 5-week-old adult females	141 761	31	31	31	31	1.02	0.43

^aThe operational taxonomic units (OTUs) were defined with pairwise 97% ID.