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. 2012 Nov 12;2:799. doi: 10.1038/srep00799

Figure 1. ER stress and chronic inflammation occurs in adipose tissue of mice fed a high-fat diet.

Figure 1

Mice were fed a regular diet or high-fat diet for 16 weeks. A, Body weight. Mice fed a high-fat diet had higher weights compared with mice fed a regular diet. Values are mean ± SD (n = 12). RD, regular diet; HFD, high-fat diet. B, Bip mRNA expression in adipose tissue of mice fed a regular diet or a high-fat diet. Bip mRNA expression increased from 12 weeks in mice fed a high-fat diet. The expression of Bip is shown relative to Gapdh. Values are mean ± SD (n = 4). **p < 0.01 (unpaired Student's t-test). C, mRNA expression levels of ER stress markers in adipose tissue. mRNA expression levels of each ER stress marker were significantly up-regulated in mice fed a high-fat diet for 16 weeks. Values are mean ± SD (n = 4). *p < 0.05 and **p < 0.01 (unpaired Student's t-test). D, mRNA expression levels of inflammatory cytokines in adipose tissue. mRNA expression levels of inflammatory cytokines were significantly up-regulated in mice fed a high-fat diet for 16 weeks. Values are mean ± SD (n = 4). *p < 0.05 and ***p < 0.001 (unpaired Student's t-test). E, H&E staining of adipose tissue. Macrophage infiltration and adipocyte hypertrophy were observed in mice fed a high-fat diet for 16 weeks.