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. Author manuscript; available in PMC: 2012 Nov 12.
Published in final edited form as: Ann Rheum Dis. 2012 Jan 17;71(4):613–616. doi: 10.1136/ard.2011.200504

Figure 1. Adult (9M) SirT1 +/− mice possess enhanced OA as compared to WT mice.

Figure 1

A. photo of 3 week littermates of the 129/J strain. B. Photo of young (1M) and adult (9M) SirT1 +/− vs. WT (SirT1 +/+) mice. Lower panel of B present immunoblots of freshly isolated articular chondrocytes from each genotype, probed with the N-SirT1 antibody. C. Left panel depicts SirT1 +/− vs. WT articular cartilage sections from the medial tibial plateau stained with Safranin O (1M: upper and 9M: lower micrographs) at x20 magnification. Adult mice (9M) were blindly ranked for OA (right panel), based on the OARSI semi-quantitative scoring for mice (12). HET denotes haploinsufficient SirT1+/− and WT denotes SirT1+/+ mice. In the graph, MTP indicates the medial tibial plateau. Black arrows in the micrographs indicate MTP of each representative section. D. Immunocytochemistry for collagen 2α1 (Col2a1; upper panels) and aggrecan (lower panels) in adult (9M) mice. Right upper graph exhibits densitometry for Col2a1 level in the extracellular matrix, while the lower right graph exhibit densitometry for aggrecan levels in murine articular chondrocytes. A.U denotes arbitrary units of intensity. Black arrow in SirT1+/− aggrecan staining, point towards irregular enlarged cell clusters. Black bars represent SirT1 +/− and white bars represent WT mice. Bars represent a mean and standard deviation of n=6 (with 2 repetitions per sample). Arrows in the immunoblots indicate the FLSirT1 band (110kD).