Figure 5.

Oxaliplatin pretreatment increases the AITC-evoked Ca²⁺response in cultured DRG neurons. The cultured neurons were pretreated with vehicle ( A) or oxaliplatin at concentrations of 30 ( B), 100 ( C) or 300 μM ( D) for 2 h after which AITC (10 μM) was added for 3 min. Representative image of fluorescence (upper panels) and Ca²⁺ concentration (F₃₄₀/F₃₈₀ ratio) recorded in individual cultured DRG neuron (lower panels). All neurons in the fields were identified based on the [Ca²⁺]_i increase elicited by the application of 50 mM KCl. Cell numbers in (AITC-sensitive DRG neurons)/(Total counted neurons) in the representative data are indicated in parentheses in lower panels.