Figure 5.
GUS staining of GL1p-GUS plants grown on MSAR medium in the presence of uniconazol. A, Plant grown on MSAR medium for 17 d showing a strong GUS staining throughout the third pair of leaves and younger leaf primordia at the center of the rosette. The second leaf pair shows staining mainly in trichomes. Note the preferential trichome staining in the second leaf pair. The first pair of leaves bear trichomes that are weakly or no longer stained. B, Plant grown on MSAR medium plus 10−6 m uniconazol for 17 d showing some staining in the third pair of leaves. Note the absence of staining in trichomes of the second leaf pair. Staining of young leaf primordia at the center of the rosette is still visible. The development of the plant was slowed by uniconazol, hence the smaller size of the plant compared with A. C, Plant grown on MSAR medium plus 10−6 m uniconazol for 13 d and transferred on MSAR medium plus 10−6 m uniconazol plus 10−5 m GAs for 4 d showing that the absence of staining in B can be specifically reversed when exogenous GAs are supplied. The third leaf pair and a second leaf pair are strongly stained, together with young primordia, at the center of the rosette. The numbers indicate the position of leaf pairs. c, Cotyledons. Bars represent 10 mm.