Figure 1. Northern analyses of tRFs. (A) Analysis of fragments derived from tRNA^Gln (CTG). RNA was extracted from cells grown under high temperature conditions [48.5°C; at exponential (lane e) and stationary phase (lane st)], separated on denaturing PAGE, transferred to membranes and hybridized with a probe against the 3′ half of tRNA^Gln (CTG) (including the terminal CCA triplet). The mature tRNA is detected with this probe as well as a tRNA derived fragment of about 40 nucleotides. A DNA size marker is given at the left in nucleotides (note that DNA runs approx. 10% faster than RNA on denaturing PAGE). (B) Analysis of fragments derived from tRNA^His (GTG). RNA was extracted from cells grown under low salt conditions (15% salt concentration in the medium; stationary phase), separated on denaturing PAGE, transferred to membranes, and hybridized with a probe against the 3′ trailer of the tRNA^His precursor. The tRNA precursor (including the tRNA and the 3′ trailer) is detected with this probe (RNA of about 120 nucleotides) as well as the 3′ trailer of about 60 nucleotides. A DNA size marker is given on the left in nucleotides (note that DNA runs approx. 10% faster than RNA on denaturing PAGE).