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. 2012 Jul 1;9(7):1011–1018. doi: 10.4161/rna.20826

graphic file with name rna-9-1011-g3.jpg

Figure 3. Deletion of hts10 gene. (A) The genomic localizations of the wild type gene and the deletion for the sRNA gene are shown schematically. The probe used for Southern blot analysis is indicated by a bar above the genome. Relevant SalI restriction sites are indicated by vertical arrows, and sizes of resulting SalI fragments are indicated below in base pairs. (B) Southern blot for deletion strain Δhts10. Lane wt: wild type, lanes 1, 2, 3: deletion strains, sizes of the DNA marker are given in base pairs at the right.