Figure 5. Genome-wide target predictions for 25 sRNAs to evaluate different constraints on the interaction seeds. The prediction performance of the tool IntaRNA using four different parameter settings is compared with the tool TargetRNA. The ROC-like plot shows the overall number of correctly predicted targets (y-axis) vs. the number of predictions per sRNA (x-axis) sorted by (energy) score. All IntaRNA predictions with constraints on the seed region (orange, light and dark blue lines) achieved a higher sensitivity (true positive rate) than IntaRNA without seed constraints (black line). Independent of the parameter setting used, IntaRNA always clearly outperformed TargetRNA (gray line).