Figure 6.

Phylogenetic tree of plant AATs. Clustering of 63 Arabidopsis, 80 rice and 59 unique barley sequences with H. vulgare phosphate transporter 1 (HvPT1) as outgroup. Colours indicate membership to different subgroups of ATF (green) and APC (blue) families, members of the aromatic amino acid transporters are shown in orange. Full-length barley sequences are given in brackets (total number/new from RNA-seq), functionally characterized transporters are given in square brackets and mentioned in Additional file 2: Table S2. Sequences from Arabidopsis and rice, including their respective nomenclature, were extracted from Aramemnon, barley sequences derived from RNA-seq, H35 (only full-length sequences), publications (HvProT, HvProT2) and previous unpublished work (HvAAP1 + 2) - see also Additional file 2: Table S2. The phylogenetic tree was constructed using the neighbor-joining algorithm in the program PAUP* [78]. The tree was displayed and manipulated using FigTree [79]. Clustering of AAT sequences into different subgroups is supported by the sequence distance matrices (Additional file 3: Figure S2). Detailed version of the phylogenetic tree including ID-numbers of all sequences is given in Additional file 1: Figure S1.